TY - JOUR

T1 - Detection of Chlamydia in postmortal formalin-fixed tissue

AU - Lundemose, A G

AU - Banner, Jytte

AU - Birkelund, Svend

AU - Christiansen, G

PY - 1989/1

Y1 - 1989/1

N2 - A procedure to detect Chlamydia in postmortal formalin-fixed tissue is described. Monoclonal antibodies against a genus specific chlamydia epitope were used in immunofluorescence to detect chlamydia inclusions in formalin-fixed tissue sections. Lung sections from chlamydia-infected mice were examined and the effect of autolysis and tetracycline treatment was evaluated. Furthermore, lung tissue from two patients who died of ornithosis was examined. Inclusions detected in lung sections showed a bright apple-green fluorescence, and had a characteristic and easily recognizable morphology. Background and non-specific fluorescence were reduced by treating the tissue sections with trypsin, rabbit serum and Evans blue counterstain. Besides giving an exact diagnosis at autopsy, the method provides the possibility of determining the occurrence of chlamydia infections in various tissues, based on retrospective investigations in formalin-fixed tissues.

AB - A procedure to detect Chlamydia in postmortal formalin-fixed tissue is described. Monoclonal antibodies against a genus specific chlamydia epitope were used in immunofluorescence to detect chlamydia inclusions in formalin-fixed tissue sections. Lung sections from chlamydia-infected mice were examined and the effect of autolysis and tetracycline treatment was evaluated. Furthermore, lung tissue from two patients who died of ornithosis was examined. Inclusions detected in lung sections showed a bright apple-green fluorescence, and had a characteristic and easily recognizable morphology. Background and non-specific fluorescence were reduced by treating the tissue sections with trypsin, rabbit serum and Evans blue counterstain. Besides giving an exact diagnosis at autopsy, the method provides the possibility of determining the occurrence of chlamydia infections in various tissues, based on retrospective investigations in formalin-fixed tissues.

KW - Animals

KW - Antibodies, Monoclonal

KW - Autopsy

KW - Chlamydia

KW - Chlamydia Infections

KW - Fluorescent Antibody Technique

KW - Formaldehyde

KW - Humans

KW - Lipopolysaccharides

KW - Lung

KW - Mice

KW - Paraffin

M3 - Journal article

C2 - 2643978

VL - 97

SP - 68

EP - 74

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 1

ER -