Extracellular calcium sensing in rat aortic vascular smooth muscle cells

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Standard

Extracellular calcium sensing in rat aortic vascular smooth muscle cells. / Smajilovic, Sanela; Hansen, Jakob Lerche; Christoffersen, Tue E.H.; Lewin, Ewa; Sheikh, Søren P.; Terwilliger, E. F.; Brown, Edward M.; Haunso, Stig; Tfelt-Hansen, Jacob.

I: Biochemical and Biophysical Research Communications, Bind 348, Nr. 4, 06.10.2006, s. 1215-1223.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Smajilovic, S, Hansen, JL, Christoffersen, TEH, Lewin, E, Sheikh, SP, Terwilliger, EF, Brown, EM, Haunso, S & Tfelt-Hansen, J 2006, 'Extracellular calcium sensing in rat aortic vascular smooth muscle cells', Biochemical and Biophysical Research Communications, bind 348, nr. 4, s. 1215-1223. https://doi.org/10.1016/j.bbrc.2006.07.192

APA

Smajilovic, S., Hansen, J. L., Christoffersen, T. E. H., Lewin, E., Sheikh, S. P., Terwilliger, E. F., Brown, E. M., Haunso, S., & Tfelt-Hansen, J. (2006). Extracellular calcium sensing in rat aortic vascular smooth muscle cells. Biochemical and Biophysical Research Communications, 348(4), 1215-1223. https://doi.org/10.1016/j.bbrc.2006.07.192

Vancouver

Smajilovic S, Hansen JL, Christoffersen TEH, Lewin E, Sheikh SP, Terwilliger EF o.a. Extracellular calcium sensing in rat aortic vascular smooth muscle cells. Biochemical and Biophysical Research Communications. 2006 okt. 6;348(4):1215-1223. https://doi.org/10.1016/j.bbrc.2006.07.192

Author

Smajilovic, Sanela ; Hansen, Jakob Lerche ; Christoffersen, Tue E.H. ; Lewin, Ewa ; Sheikh, Søren P. ; Terwilliger, E. F. ; Brown, Edward M. ; Haunso, Stig ; Tfelt-Hansen, Jacob. / Extracellular calcium sensing in rat aortic vascular smooth muscle cells. I: Biochemical and Biophysical Research Communications. 2006 ; Bind 348, Nr. 4. s. 1215-1223.

Bibtex

@article{a0289744d3de4825855ebb15738a70c5,
title = "Extracellular calcium sensing in rat aortic vascular smooth muscle cells",
abstract = "Extracellular calcium (Ca2 + o) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca2 + o stimulates proliferation of the cells. The effects of Ca2 + o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca2 + o-induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca2 + o-induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium.",
keywords = "Atherosclerosis, Calcium-sensing receptor, ERK1/2, Proliferation, Vascular smooth muscle cell",
author = "Sanela Smajilovic and Hansen, {Jakob Lerche} and Christoffersen, {Tue E.H.} and Ewa Lewin and Sheikh, {S{\o}ren P.} and Terwilliger, {E. F.} and Brown, {Edward M.} and Stig Haunso and Jacob Tfelt-Hansen",
year = "2006",
month = oct,
day = "6",
doi = "10.1016/j.bbrc.2006.07.192",
language = "English",
volume = "348",
pages = "1215--1223",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Extracellular calcium sensing in rat aortic vascular smooth muscle cells

AU - Smajilovic, Sanela

AU - Hansen, Jakob Lerche

AU - Christoffersen, Tue E.H.

AU - Lewin, Ewa

AU - Sheikh, Søren P.

AU - Terwilliger, E. F.

AU - Brown, Edward M.

AU - Haunso, Stig

AU - Tfelt-Hansen, Jacob

PY - 2006/10/6

Y1 - 2006/10/6

N2 - Extracellular calcium (Ca2 + o) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca2 + o stimulates proliferation of the cells. The effects of Ca2 + o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca2 + o-induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca2 + o-induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium.

AB - Extracellular calcium (Ca2 + o) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca2 + o stimulates proliferation of the cells. The effects of Ca2 + o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca2 + o-induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca2 + o-induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium.

KW - Atherosclerosis

KW - Calcium-sensing receptor

KW - ERK1/2

KW - Proliferation

KW - Vascular smooth muscle cell

UR - http://www.scopus.com/inward/record.url?scp=33748147021&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.07.192

DO - 10.1016/j.bbrc.2006.07.192

M3 - Journal article

C2 - 16919596

AN - SCOPUS:33748147021

VL - 348

SP - 1215

EP - 1223

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

ID: 203876081