The AmpFℓSTR® NGM SElect™ Kit is a highly robust multiplex kit developed for human identification. This kit contains the expanded European Standard Set of Loci and the highly polymorphic SE33 locus. The high power of discrimination makes it one of the most informative kits for forensic applications. In order to evaluate the kit and get population data for Chinese Uyghurs and Kazakhs, the genetic polymorphisms of 16 autosomal STR loci included in the NGM SElect™ Kit: D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D10S1248, D22S1045, D2S441, D1S1656 D12S391, SE33, and Amelogenin were studied in 324 unrelated Uyghur and 124 unrelated Kazakh individuals from the Xinjiang Autonomous Region of China. Allele frequencies and forensic relevant parameters were calculated using the Arlequin software and DNAVIEW. The genotype frequency distribution of each locus did not deviate from Hardy-Weinberg Equilibrium in any of the two populations. The forensic parameters indicated that the kit is suitable for personal identification, paternity testing, and complex kinship analysis in Uyghur and Kazakh populations. Allele frequency data for the STR loci were compared with other previously published population data.