TY - JOUR

T1 - The EUROFORGEN NAME Ampliseq™ custom panel

T2 - A second tier panel developed for differentiation of individuals from the Middle East/North Africa

AU - Truelsen, D. M.

AU - Pereira, V.

AU - Phillips, C.

AU - Morling, N.

AU - Børsting, C.

PY - 2019/12

Y1 - 2019/12

N2 - The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq™ custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5™ (Thermo Fisher Scientific). Of the 111 SNPs selected in the first version of the panel, 107 SNPs were successfully incorporated in the AmpliSeq™ multiplex PCR. More than 600 individuals from 13 different countries in the Middle East, North Africa, and Europe were typed. The performance of the sequencing assay was assessed by calculating relative locus balances, heterozygote balances, and noise levels. Lastly, likelihood ratios were calculated using the GenoGeographer software.

AB - The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq™ custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5™ (Thermo Fisher Scientific). Of the 111 SNPs selected in the first version of the panel, 107 SNPs were successfully incorporated in the AmpliSeq™ multiplex PCR. More than 600 individuals from 13 different countries in the Middle East, North Africa, and Europe were typed. The performance of the sequencing assay was assessed by calculating relative locus balances, heterozygote balances, and noise levels. Lastly, likelihood ratios were calculated using the GenoGeographer software.

KW - Ancestry informative markers

KW - EUROFORGEN NAME ancestry panel

KW - Ion AmpliSeq™ custom panel

KW - Ion S5™ sequencing

KW - NAME

KW - Second-tier panel

U2 - 10.1016/j.fsigss.2019.10.199

DO - 10.1016/j.fsigss.2019.10.199

M3 - Journal article

AN - SCOPUS:85075369835

VL - 7

SP - 846

EP - 848

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

SN - 1875-1768

IS - 1

ER -