The Karimojong from Uganda: genetic characterization using an X-STR decaplex system
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The Karimojong from Uganda : genetic characterization using an X-STR decaplex system. / Gomes, Iva; Pereira, Vania; Gomes, Verónica; Prata, Maria João; Pinto, Nádia; Carracedo, Angel; Amorim, António; Gusmão, Leonor.
I: Forensic science international. Genetics, Bind 3, Nr. 4, 09.2009, s. e127-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The Karimojong from Uganda
T2 - genetic characterization using an X-STR decaplex system
AU - Gomes, Iva
AU - Pereira, Vania
AU - Gomes, Verónica
AU - Prata, Maria João
AU - Pinto, Nádia
AU - Carracedo, Angel
AU - Amorim, António
AU - Gusmão, Leonor
PY - 2009/9
Y1 - 2009/9
N2 - The Karimojong, an African group from the Karamoja region of Northeast Uganda, were genetically analysed using a decaplex system for X chromosome short tandem repeats (X-STRs). A total of 255 individuals (117 males and 138 females) were genotyped for the following loci: DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS7423, DXS6809, DXS7132, DXS9902 and DXS6789. Allele frequencies and parameters for forensic evaluation were calculated for each STR. No association was found between any pairs of loci studied. DXS6789 was the most polymorphic marker in this sample, followed by DXS6809, with gene diversities of 84.79% and 83.94%, respectively. The less discriminating locus observed was DXS7133, with a gene diversity of 39.79%. High overall values of power of discrimination were obtained for female (1 in 1.8 x 10(10)) and male samples (1 in 1.6 x 10(6)), as well as high power of exclusion in father/mother/daughter trios (99.9997%), in father daughter duos (99.9862%) and in half sisters with same father (99.0331%). These results confirm the potential of this 10-plex in parentage testing and in human identification.
AB - The Karimojong, an African group from the Karamoja region of Northeast Uganda, were genetically analysed using a decaplex system for X chromosome short tandem repeats (X-STRs). A total of 255 individuals (117 males and 138 females) were genotyped for the following loci: DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS7423, DXS6809, DXS7132, DXS9902 and DXS6789. Allele frequencies and parameters for forensic evaluation were calculated for each STR. No association was found between any pairs of loci studied. DXS6789 was the most polymorphic marker in this sample, followed by DXS6809, with gene diversities of 84.79% and 83.94%, respectively. The less discriminating locus observed was DXS7133, with a gene diversity of 39.79%. High overall values of power of discrimination were obtained for female (1 in 1.8 x 10(10)) and male samples (1 in 1.6 x 10(6)), as well as high power of exclusion in father/mother/daughter trios (99.9997%), in father daughter duos (99.9862%) and in half sisters with same father (99.0331%). These results confirm the potential of this 10-plex in parentage testing and in human identification.
KW - African Continental Ancestry Group
KW - Alleles
KW - Chromosomes, Human, X
KW - DNA
KW - Female
KW - Forensic Genetics
KW - Gene Frequency
KW - Genetic Variation
KW - Genetics, Population
KW - Genotype
KW - Humans
KW - Male
KW - Microsatellite Repeats
KW - Polymerase Chain Reaction
KW - Quality Control
KW - Uganda
U2 - 10.1016/j.fsigen.2008.10.009
DO - 10.1016/j.fsigen.2008.10.009
M3 - Journal article
C2 - 19647697
VL - 3
SP - e127-8
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
SN - 1872-4973
IS - 4
ER -
ID: 46232653