A biocompatible micro cell culture chamber (microCCC) for the culturing and on-line monitoring of eukaryote cells
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A biocompatible micro cell culture chamber (microCCC) for the culturing and on-line monitoring of eukaryote cells. / Stangegaard, Michael; Petronis, Sarunas; Jørgensen, A M; Christensen, C B V; Dufva, Martin.
In: Lab On a Chip, Vol. 6, No. 8, 08.2006, p. 1045-51.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A biocompatible micro cell culture chamber (microCCC) for the culturing and on-line monitoring of eukaryote cells
AU - Stangegaard, Michael
AU - Petronis, Sarunas
AU - Jørgensen, A M
AU - Christensen, C B V
AU - Dufva, Martin
PY - 2006/8
Y1 - 2006/8
N2 - We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.
AB - We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.
KW - Caco-2 Cells
KW - Cell Culture Techniques
KW - Cell Survival
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Hela Cells
KW - Humans
KW - Microfluidic Analytical Techniques
KW - Reverse Transcriptase Polymerase Chain Reaction
U2 - 10.1039/b603379b
DO - 10.1039/b603379b
M3 - Journal article
C2 - 16874376
VL - 6
SP - 1045
EP - 1051
JO - Lab on a Chip
JF - Lab on a Chip
SN - 1473-0197
IS - 8
ER -
ID: 32443687