Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry. / Rück, Vanessa; Mishra, Narendra K.; Sørensen, Kasper K.; Liisberg, Mikkel B.; Sloth, Ane B.; Cerretani, Cecilia; Mollerup, Christian B.; Kjaer, Andreas; Lou, Chenguang; Jensen, Knud J.; Vosch, Tom.

In: Journal of the American Chemical Society, Vol. 145, No. 30, 2023, p. 16771–16777.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rück, V, Mishra, NK, Sørensen, KK, Liisberg, MB, Sloth, AB, Cerretani, C, Mollerup, CB, Kjaer, A, Lou, C, Jensen, KJ & Vosch, T 2023, 'Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry', Journal of the American Chemical Society, vol. 145, no. 30, pp. 16771–16777. https://doi.org/10.1021/jacs.3c04768

APA

Rück, V., Mishra, N. K., Sørensen, K. K., Liisberg, M. B., Sloth, A. B., Cerretani, C., Mollerup, C. B., Kjaer, A., Lou, C., Jensen, K. J., & Vosch, T. (2023). Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry. Journal of the American Chemical Society, 145(30), 16771–16777. https://doi.org/10.1021/jacs.3c04768

Vancouver

Rück V, Mishra NK, Sørensen KK, Liisberg MB, Sloth AB, Cerretani C et al. Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry. Journal of the American Chemical Society. 2023;145(30):16771–16777. https://doi.org/10.1021/jacs.3c04768

Author

Rück, Vanessa ; Mishra, Narendra K. ; Sørensen, Kasper K. ; Liisberg, Mikkel B. ; Sloth, Ane B. ; Cerretani, Cecilia ; Mollerup, Christian B. ; Kjaer, Andreas ; Lou, Chenguang ; Jensen, Knud J. ; Vosch, Tom. / Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry. In: Journal of the American Chemical Society. 2023 ; Vol. 145, No. 30. pp. 16771–16777.

Bibtex

@article{c89afdde806546baba69e86799c208b9,
title = "Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry",
abstract = "DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.",
author = "Vanessa R{\"u}ck and Mishra, {Narendra K.} and S{\o}rensen, {Kasper K.} and Liisberg, {Mikkel B.} and Sloth, {Ane B.} and Cecilia Cerretani and Mollerup, {Christian B.} and Andreas Kjaer and Chenguang Lou and Jensen, {Knud J.} and Tom Vosch",
note = "Funding Information: V.R., M.B.L., C.C., and T.V. acknowledge funding from the Villum Foundation (VKR023115) and the Independent Research Fund Denmark (0136-00024B). N.K.M., K.K.S., and K.J.J. thank the Villum Foundation (VKR18333) for funding the Biomolecular Nanoscale Engineering Center (BioNEC), a VILLUM center of excellence, and the Novo Nordisk Foundation funding for the Center for Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery; Grand Challenge Program; NNF16OC0021948). The authors would like to thank Morten Lundh from Gubra for providing the CHO cells expressing the hIR-B. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",
year = "2023",
doi = "10.1021/jacs.3c04768",
language = "English",
volume = "145",
pages = "16771–16777",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "ACS Publications",
number = "30",

}

RIS

TY - JOUR

T1 - Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry

AU - Rück, Vanessa

AU - Mishra, Narendra K.

AU - Sørensen, Kasper K.

AU - Liisberg, Mikkel B.

AU - Sloth, Ane B.

AU - Cerretani, Cecilia

AU - Mollerup, Christian B.

AU - Kjaer, Andreas

AU - Lou, Chenguang

AU - Jensen, Knud J.

AU - Vosch, Tom

N1 - Funding Information: V.R., M.B.L., C.C., and T.V. acknowledge funding from the Villum Foundation (VKR023115) and the Independent Research Fund Denmark (0136-00024B). N.K.M., K.K.S., and K.J.J. thank the Villum Foundation (VKR18333) for funding the Biomolecular Nanoscale Engineering Center (BioNEC), a VILLUM center of excellence, and the Novo Nordisk Foundation funding for the Center for Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery; Grand Challenge Program; NNF16OC0021948). The authors would like to thank Morten Lundh from Gubra for providing the CHO cells expressing the hIR-B. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023

Y1 - 2023

N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.

AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.

U2 - 10.1021/jacs.3c04768

DO - 10.1021/jacs.3c04768

M3 - Journal article

C2 - 37441791

AN - SCOPUS:85165644291

VL - 145

SP - 16771

EP - 16777

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 30

ER -

ID: 361438788