TY - JOUR

T1 - Cleaning protocols in forensic genetic laboratories

AU - Kampmann, Marie-Louise

AU - Tfelt-Hansen, Jacob

AU - Børsting, Claus

N1 - © 2024. The Author(s).

PY - 2024

Y1 - 2024

N2 - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD 4L did not.

AB - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD 4L did not.

U2 - 10.1007/s00414-024-03232-0

DO - 10.1007/s00414-024-03232-0

M3 - Journal article

C2 - 38649547

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

SN - 0937-9827

ER -