Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories
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Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories. / Kampmann, Marie Louise; Simonsen, Bo Thisted; Børsting, Claus.
In: Forensic Science International: Genetics Supplement Series, Vol. 8, 2022, p. 149-150.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Test of chlorine wipes for efficient removal of DNA from forensic genetics laboratories
AU - Kampmann, Marie Louise
AU - Simonsen, Bo Thisted
AU - Børsting, Claus
N1 - Funding Information: The authors thank Anja Jørgensen for technical assistance. Publisher Copyright: © 2022 The Authors
PY - 2022
Y1 - 2022
N2 - Sodium hypochlorite is an efficient reagent for removal of unwanted DNA from laboratory surfaces. Here, we tested two different chlorine wipes and compared their performance to a 0.9–1.8% hypochlorite solution. WipeClean Chlorine Disinfection wipes contain > 0.1 g sodium hypochlorite/kg, whereas WetWipe Chlorine Desinfection wipes contain > 1000 ppm active chlorine. Clean surfaces were contaminated with 10 µL 0.5 ng/µL of massively parallel sequencing libraries. The DNA was dried and left for 45 min before any treatment. The surfaces were cleaned using either 1) a 0.9–1.8% hypochlorite solution and clean wipes, 2) a WipeClean wipe, 3) a WetWipe, or 4) the surface was not cleaned. All experiments were repeated three times. Subsequently, the surfaces were swabbed using cotton swabs. DNA was extracted from the swabs and the DNA concentrations were determined in quadruplicates by real-time PCR. This protocol was repeated after the soft plastic wrapping around the wipes were left open or closed for several weeks. The results showed that the WipeClean wipes efficiently removed DNA for up to four weeks after the box with the wipes were opened, whereas the WetWipe wipes dried faster and gradually lost their cleaning effect.
AB - Sodium hypochlorite is an efficient reagent for removal of unwanted DNA from laboratory surfaces. Here, we tested two different chlorine wipes and compared their performance to a 0.9–1.8% hypochlorite solution. WipeClean Chlorine Disinfection wipes contain > 0.1 g sodium hypochlorite/kg, whereas WetWipe Chlorine Desinfection wipes contain > 1000 ppm active chlorine. Clean surfaces were contaminated with 10 µL 0.5 ng/µL of massively parallel sequencing libraries. The DNA was dried and left for 45 min before any treatment. The surfaces were cleaned using either 1) a 0.9–1.8% hypochlorite solution and clean wipes, 2) a WipeClean wipe, 3) a WetWipe, or 4) the surface was not cleaned. All experiments were repeated three times. Subsequently, the surfaces were swabbed using cotton swabs. DNA was extracted from the swabs and the DNA concentrations were determined in quadruplicates by real-time PCR. This protocol was repeated after the soft plastic wrapping around the wipes were left open or closed for several weeks. The results showed that the WipeClean wipes efficiently removed DNA for up to four weeks after the box with the wipes were opened, whereas the WetWipe wipes dried faster and gradually lost their cleaning effect.
KW - Chlorine wipes
KW - DNA
KW - Hypochlorite
KW - Laboratory contamination
KW - PCR
U2 - 10.1016/j.fsigss.2022.10.016
DO - 10.1016/j.fsigss.2022.10.016
M3 - Journal article
AN - SCOPUS:85139631054
VL - 8
SP - 149
EP - 150
JO - Forensic Science International: Genetics. Supplement Series
JF - Forensic Science International: Genetics. Supplement Series
SN - 1875-1768
ER -
ID: 323297186