Cleaning protocols in forensic genetic laboratories
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Cleaning protocols in forensic genetic laboratories. / Kampmann, Marie-Louise; Tfelt-Hansen, Jacob; Børsting, Claus.
In: International Journal of Legal Medicine, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cleaning protocols in forensic genetic laboratories
AU - Kampmann, Marie-Louise
AU - Tfelt-Hansen, Jacob
AU - Børsting, Claus
N1 - © 2024. The Author(s).
PY - 2024
Y1 - 2024
N2 - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD 4L did not.
AB - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD 4L did not.
U2 - 10.1007/s00414-024-03232-0
DO - 10.1007/s00414-024-03232-0
M3 - Journal article
C2 - 38649547
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
SN - 0937-9827
ER -
ID: 389849417