Hybridization capture using short PCR products enriches small genomes by Capturing Flanking Sequences (CapFlank)
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Tsangaras_2014_Hybridization_capture
Final published version, 876 KB, PDF document
Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. We demonstrate that during capture, the bait-hybridized library molecules add additional flanking library sequences iteratively, such that baits limited to targeting relatively short regions (e.g. few hundred nucleotides) can result in enrichment across entire mitochondrial and bacterial genomes. Our findings suggest that some of the off-target sequences derived in capture experiments are non-randomly enriched, and that CapFlank will facilitate targeted enrichment of large contiguous sequences with minimal prior target sequence information.
Original language | English |
---|---|
Article number | e109101 |
Journal | PLoS ONE |
Volume | 9 |
Issue number | 10 |
Number of pages | 10 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2014 |
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 129542559