Simultaneous determination of gamma-Hydroxybutyrate (GHB) and its analogues (GBL, 1.4-BD, GVL) in whole blood and urine by liquid chromatography coupled to tandem mass spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
A simple liquid chromatography-tandem mass spectrometry (LC-MS-MS) method has been developed and validated for simultaneous identification and quantification of ¿-hydroxybutyrate (GHB), ¿-butyrolactone (GBL), 1.4-butanediol (1.4-BD), and ¿-valerolactone (GVL) in whole blood from forensic cases. The sample preparation of whole blood involved protein precipitation by acidic methanol. Urine samples were diluted and evaluated in relation to a control at the cutoff concentration. Hexadeutero GHB (GHB-d(6)) was used as the internal standard. Separation was achieved by reversed-phase chromatography, and detection was by MS-MS in MRM mode. The linear range for all compounds was from 1.0 to 100 mg/kg in whole blood with a limit of quantification of about 1 mg/kg. The method was validated with regards to selectivity, recovery, accuracy and precision, and stability. The method is currently applied to investigations on suspected drug-facilitated sexual assaults, driving under the influence of drugs, and general intoxication with these substances.
Original language | English |
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Journal | Journal of Analytical Toxicology |
Volume | 35 |
Issue number | 1 |
Pages (from-to) | 8-14 |
Number of pages | 7 |
ISSN | 0146-4760 |
Publication status | Published - 2011 |
- 4-Butyrolactone, Butylene Glycols, Chromatography, Liquid, Chromatography, Reverse-Phase, Gas Chromatography-Mass Spectrometry, Humans, Lactones, Sodium Oxybate, Tandem Mass Spectrometry
Research areas
ID: 38550930