A multiplex assay with 52 single nucleotide polymorphisms for human identification
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A multiplex assay with 52 single nucleotide polymorphisms for human identification. / Sanchez Sanchez, Juan Jose; Phillips, Chris; Børsting, Claus; Balogh, Kinga; Bogus, Magdalena; Fondevila, Manuel; Harrison, Cheryl D; Musgrave-Brown, Esther; Salas, Antonio; Syndercombe-Court, Denise; Schneider, Peter M; Carracedo, Angel; Morling, Niels.
In: Electrophoresis, Vol. 27, No. 9, 2006, p. 1713-24.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A multiplex assay with 52 single nucleotide polymorphisms for human identification
AU - Sanchez Sanchez, Juan Jose
AU - Phillips, Chris
AU - Børsting, Claus
AU - Balogh, Kinga
AU - Bogus, Magdalena
AU - Fondevila, Manuel
AU - Harrison, Cheryl D
AU - Musgrave-Brown, Esther
AU - Salas, Antonio
AU - Syndercombe-Court, Denise
AU - Schneider, Peter M
AU - Carracedo, Angel
AU - Morling, Niels
N1 - Keywords: Continental Population Groups; DNA Fingerprinting; DNA Primers; Forensic Medicine; Gene Frequency; Humans; Paternity; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Sequence Analysis, DNA
PY - 2006
Y1 - 2006
N2 - A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org.
AB - A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org.
U2 - 10.1002/elps.200500671
DO - 10.1002/elps.200500671
M3 - Journal article
C2 - 16586411
VL - 27
SP - 1713
EP - 1724
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 9
ER -
ID: 14144998