Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem

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Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem. / Rohde, Marianne Cathrine; Corydon, Thomas Juhl; Hansen, Jakob; Pedersen, Christina Bak; Schmidt, Stinne P.; Gregersen, Niels; Banner, Jytte.

I: Forensic Science International, Bind 234, 01.2014, s. 149-53.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rohde, MC, Corydon, TJ, Hansen, J, Pedersen, CB, Schmidt, SP, Gregersen, N & Banner, J 2014, 'Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem', Forensic Science International, bind 234, s. 149-53. https://doi.org/10.1016/j.forsciint.2013.11.007

APA

Rohde, M. C., Corydon, T. J., Hansen, J., Pedersen, C. B., Schmidt, S. P., Gregersen, N., & Banner, J. (2014). Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem. Forensic Science International, 234, 149-53. https://doi.org/10.1016/j.forsciint.2013.11.007

Vancouver

Rohde MC, Corydon TJ, Hansen J, Pedersen CB, Schmidt SP, Gregersen N o.a. Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem. Forensic Science International. 2014 jan.;234:149-53. https://doi.org/10.1016/j.forsciint.2013.11.007

Author

Rohde, Marianne Cathrine ; Corydon, Thomas Juhl ; Hansen, Jakob ; Pedersen, Christina Bak ; Schmidt, Stinne P. ; Gregersen, Niels ; Banner, Jytte. / Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem. I: Forensic Science International. 2014 ; Bind 234. s. 149-53.

Bibtex

@article{660d255ae31e4a649d0db3b475d87ca5,

title = "Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem",

abstract = "Primary cell cultures were investigated as a tool for molecular diagnostics in a forensic setting. Fibroblast cultures had been established from human Achilles tendon resected at autopsies, from cases of sudden infant death syndrome and control infants who died in traumatic events (n=41). After isolation of primary cultures cells were stored at -135°C, and re-established up to 15 years later for experimental intervention. Growth characteristics in cultures were evaluated in relation to the age of the donor, the post mortem interval before sampling, and the storage interval of cells before entry into the study. High interpersonal variation in growth rates and cell doubling time was seen, but no statistically significant differences were found with increasing age of the donor (mean 19 weeks), length of post-mortem interval prior to sampling (6-100h), or increase in years of storage. Fibroblast cultures established from post-mortem tissue are renewable sources of biological material; they can be the foundation for genetic, metabolic and other functional studies and thus constitute a valuable tool for molecular and pathophysiological investigations in biomedical and forensic sciences.",

author = "Rohde, {Marianne Cathrine} and Corydon, {Thomas Juhl} and Jakob Hansen and Pedersen, {Christina Bak} and Schmidt, {Stinne P.} and Niels Gregersen and Jytte Banner",

year = "2014",

month = jan,

doi = "10.1016/j.forsciint.2013.11.007",

language = "English",

volume = "234",

pages = "149--53",

journal = "Forensic Science International",

issn = "0379-0738",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Characteristics of human infant primary fibroblast cultures from Achilles tendons removed post-mortem

AU - Rohde, Marianne Cathrine

AU - Corydon, Thomas Juhl

AU - Hansen, Jakob

AU - Pedersen, Christina Bak

AU - Schmidt, Stinne P.

AU - Gregersen, Niels

AU - Banner, Jytte

PY - 2014/1

Y1 - 2014/1

N2 - Primary cell cultures were investigated as a tool for molecular diagnostics in a forensic setting. Fibroblast cultures had been established from human Achilles tendon resected at autopsies, from cases of sudden infant death syndrome and control infants who died in traumatic events (n=41). After isolation of primary cultures cells were stored at -135°C, and re-established up to 15 years later for experimental intervention. Growth characteristics in cultures were evaluated in relation to the age of the donor, the post mortem interval before sampling, and the storage interval of cells before entry into the study. High interpersonal variation in growth rates and cell doubling time was seen, but no statistically significant differences were found with increasing age of the donor (mean 19 weeks), length of post-mortem interval prior to sampling (6-100h), or increase in years of storage. Fibroblast cultures established from post-mortem tissue are renewable sources of biological material; they can be the foundation for genetic, metabolic and other functional studies and thus constitute a valuable tool for molecular and pathophysiological investigations in biomedical and forensic sciences.

AB - Primary cell cultures were investigated as a tool for molecular diagnostics in a forensic setting. Fibroblast cultures had been established from human Achilles tendon resected at autopsies, from cases of sudden infant death syndrome and control infants who died in traumatic events (n=41). After isolation of primary cultures cells were stored at -135°C, and re-established up to 15 years later for experimental intervention. Growth characteristics in cultures were evaluated in relation to the age of the donor, the post mortem interval before sampling, and the storage interval of cells before entry into the study. High interpersonal variation in growth rates and cell doubling time was seen, but no statistically significant differences were found with increasing age of the donor (mean 19 weeks), length of post-mortem interval prior to sampling (6-100h), or increase in years of storage. Fibroblast cultures established from post-mortem tissue are renewable sources of biological material; they can be the foundation for genetic, metabolic and other functional studies and thus constitute a valuable tool for molecular and pathophysiological investigations in biomedical and forensic sciences.

U2 - 10.1016/j.forsciint.2013.11.007

DO - 10.1016/j.forsciint.2013.11.007

M3 - Journal article

C2 - 24378315

VL - 234

SP - 149

EP - 153

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

ER -

ID: 97247256

Retsmedicinsk Institut