The effect of whole genome amplification on samples originating from more than one donor
Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › fagfællebedømt
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The effect of whole genome amplification on samples originating from more than one donor. / Thacker, C.R.; Balogh, M.K.; Børsting, Claus; Ramos, E.; Sanchez, Diz P.; Carracedo, A.; Morling, N.; Schneider, Peter M.; Syndercombe-Court, Denise; SNPforID Consortium.
Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress. Bind 1288 Elsevier, 2006. s. 722-724 (ICS - International Congress Series).Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › fagfællebedømt
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TY - GEN
T1 - The effect of whole genome amplification on samples originating from more than one donor
AU - Thacker, C.R.
AU - Balogh, M.K.
AU - Børsting, Claus
AU - Ramos, E.
AU - Sanchez, Diz P.
AU - Carracedo, A.
AU - Morling, N.
AU - Schneider, Peter M.
AU - Syndercombe-Court, Denise
AU - SNPforID Consortium
PY - 2006
Y1 - 2006
N2 - In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained
AB - In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained
U2 - doi:10.1016/j.ics.2005.09.170
DO - doi:10.1016/j.ics.2005.09.170
M3 - Article in proceedings
VL - 1288
T3 - ICS - International Congress Series
SP - 722
EP - 724
BT - Progress in Forensic Genetics 11
PB - Elsevier
Y2 - 13 September 2005 through 16 September 2005
ER -
ID: 18251470