Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series
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Neurological Complications in COVID-19 Patients : Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. / Lauridsen, Karin Holst; Olsen, Kristine Boisen; Lund, Eva Løbner; Jensen, Tomas O.; Pedersen, Thomas Ingemann; Harboe, Zitta Barrella; Antsupova, Valeria; Rasmussen, Lasse Dam; Röser, Dennis; Banner, Jytte; Franck, Kristina Træholt; Vorobieva Solholm Jensen, Veronika.
I: Clinical Pathology, Bind 15, 2022, s. 1-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Neurological Complications in COVID-19 Patients
T2 - Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series
AU - Lauridsen, Karin Holst
AU - Olsen, Kristine Boisen
AU - Lund, Eva Løbner
AU - Jensen, Tomas O.
AU - Pedersen, Thomas Ingemann
AU - Harboe, Zitta Barrella
AU - Antsupova, Valeria
AU - Rasmussen, Lasse Dam
AU - Röser, Dennis
AU - Banner, Jytte
AU - Franck, Kristina Træholt
AU - Vorobieva Solholm Jensen, Veronika
N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: All authors were funded by their individual departments. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PY - 2022
Y1 - 2022
N2 - Background: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection. Methods and Results: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus. Conclusion: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.
AB - Background: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection. Methods and Results: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus. Conclusion: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.
KW - Antibodies
KW - autopsy
KW - case reports
KW - coronavirus
KW - immunohistochemistry
KW - SARS virus
U2 - 10.1177/2632010X221139096
DO - 10.1177/2632010X221139096
M3 - Journal article
C2 - 36448025
AN - SCOPUS:85142762469
VL - 15
SP - 1
EP - 7
JO - Clinical Pathology
JF - Clinical Pathology
SN - 2632-010X
ER -
ID: 328528372