Investigating in-sewer transformation products formed from synthetic cathinones and phenethylamines using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry
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Investigating in-sewer transformation products formed from synthetic cathinones and phenethylamines using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. / Kinyua, Juliet; Negreira, Noelia; McCall, Ann Kathrin; Boogaerts, Tim; Ort, Christoph; Covaci, Adrian; van Nuijs, Alexander L.N.
I: Science of the Total Environment, Bind 634, 2018, s. 331-340.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Investigating in-sewer transformation products formed from synthetic cathinones and phenethylamines using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry
AU - Kinyua, Juliet
AU - Negreira, Noelia
AU - McCall, Ann Kathrin
AU - Boogaerts, Tim
AU - Ort, Christoph
AU - Covaci, Adrian
AU - van Nuijs, Alexander L.N.
N1 - Funding Information: We would like to thank the staff at Toxicological Center (UA), Eawag, Swiss Federal Institute of Aquatic Science and Technology, Philanthropic Educational Organization (PEO) and American Association of University Women (AAUW) for their support. Financial support: Dr. Juliet Kinyua acknowledges the EU International Training Network SEWPROF (Marie Curie- Grant number 317205 ) for her grant. Part of this work was supported by the COST Action ES1307 “SCORE – Sewage biomarker analysis for community health assessment”. Dr. Alexander van Nuijs and Dr. Noelia Negreira acknowledge the Research Foundation Flanders (FWO) and University of Antwerp for their respective post-doctoral fellowships. Publisher Copyright: © 2018 Elsevier B.V.
PY - 2018
Y1 - 2018
N2 - Recent studies have demonstrated the role of biofilms on the stability of drug residues in wastewater. These factors are pertinent in wastewater-based epidemiology (WBE) when estimating community-level drug use. However, there is scarce information on the biotransformation of drug residues in the presence of biofilms and the potential use of transformation products (TPs) as biomarkers in WBE. The purpose of this work was to investigate the formation of TPs in sewage reactors in the presence of biofilm mimicking conditions during in-sewer transport. Synthetic cathinones (methylenedioxypyrovalerone, methylone, mephedrone) and phenethylamines (4-methoxy-methamphetamine and 4-methoxyamphetamine) were incubated in individual reactors over a 24 h period. Analysis of parent species and TPs was carried out using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QToFMS). Identification of TPs was done using suspect and non-target workflows. In total, 18 TPs were detected and identified with reduction of β-keto group, demethylenation, demethylation, and hydroxylation reactions observed for the synthetic cathinones. For the phenethylamines, N- and O-demethylation reactions were identified. Overall, the experiments showed varying stability for the parent species in wastewater in the presence of biofilms. The newly identified isomeric forms of TPs particularly for methylone and mephedrone can be used as potential target biomarkers for WBE studies due to their specificity and detectability within a 24 h residence time.
AB - Recent studies have demonstrated the role of biofilms on the stability of drug residues in wastewater. These factors are pertinent in wastewater-based epidemiology (WBE) when estimating community-level drug use. However, there is scarce information on the biotransformation of drug residues in the presence of biofilms and the potential use of transformation products (TPs) as biomarkers in WBE. The purpose of this work was to investigate the formation of TPs in sewage reactors in the presence of biofilm mimicking conditions during in-sewer transport. Synthetic cathinones (methylenedioxypyrovalerone, methylone, mephedrone) and phenethylamines (4-methoxy-methamphetamine and 4-methoxyamphetamine) were incubated in individual reactors over a 24 h period. Analysis of parent species and TPs was carried out using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QToFMS). Identification of TPs was done using suspect and non-target workflows. In total, 18 TPs were detected and identified with reduction of β-keto group, demethylenation, demethylation, and hydroxylation reactions observed for the synthetic cathinones. For the phenethylamines, N- and O-demethylation reactions were identified. Overall, the experiments showed varying stability for the parent species in wastewater in the presence of biofilms. The newly identified isomeric forms of TPs particularly for methylone and mephedrone can be used as potential target biomarkers for WBE studies due to their specificity and detectability within a 24 h residence time.
KW - Biofilm
KW - Biomarkers
KW - LC-QToFMS
KW - New psychoactive substances
KW - Stability
KW - Wastewater-based epidemiology
U2 - 10.1016/j.scitotenv.2018.03.253
DO - 10.1016/j.scitotenv.2018.03.253
M3 - Journal article
C2 - 29627557
AN - SCOPUS:85044855741
VL - 634
SP - 331
EP - 340
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
ER -
ID: 275536237