Simultaneous determination of ethanol's four types of non-oxidative metabolites in human whole blood by liquid chromatography tandem mass spectrometry
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Simultaneous determination of ethanol's four types of non-oxidative metabolites in human whole blood by liquid chromatography tandem mass spectrometry. / Zhang, Xinyu; Zheng, Feng; Lin, Zebin; Johansen, Sys Stybe; Yu, Tianfang; Liu, Yuming; Huang, Zhibin; Li, Jiaolun; Yan, Jie; Rao, Yulan.
I: Analytica Chimica Acta, Bind 963, 2017, s. 68-75.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Simultaneous determination of ethanol's four types of non-oxidative metabolites in human whole blood by liquid chromatography tandem mass spectrometry
AU - Zhang, Xinyu
AU - Zheng, Feng
AU - Lin, Zebin
AU - Johansen, Sys Stybe
AU - Yu, Tianfang
AU - Liu, Yuming
AU - Huang, Zhibin
AU - Li, Jiaolun
AU - Yan, Jie
AU - Rao, Yulan
N1 - Copyright © 2017 Elsevier B.V. All rights reserved.
PY - 2017
Y1 - 2017
N2 - The importance of ethanol non-oxidative metabolites as the specific biomarkers of alcohol consumption in clinical and forensic settings is increasingly acknowledged. Simultaneous determination of these metabolites can provide a wealth of information like drinking habit and history, but it was difficult to achieve because of their wide range of polarity. This work describes development and validation of a simple liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for 4 types of ethanol non-oxidative metabolites (ethyl glucuronide, ethyl sulfate, fatty acid ethyl esters and phosphatidylethanols) in 50 μL of human whole blood. Pretreatment method, column and MS conditions were optimized. For the first time, the four types of ethanol non-oxidative metabolites with enormous discrepancies of property were simultaneously extracted and analyzed in one run within 40 min. The limits of detections (LODs) were among 0.1-10 ng/mL, and good linearity was obtained. Deviations in precision and accuracy were all lower than 15% at three QC levels. This method was then applied to two forensic samples, resulting in information on drinking habits and drinking time which were very useful for the interpretation of the blood alcohol results.
AB - The importance of ethanol non-oxidative metabolites as the specific biomarkers of alcohol consumption in clinical and forensic settings is increasingly acknowledged. Simultaneous determination of these metabolites can provide a wealth of information like drinking habit and history, but it was difficult to achieve because of their wide range of polarity. This work describes development and validation of a simple liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for 4 types of ethanol non-oxidative metabolites (ethyl glucuronide, ethyl sulfate, fatty acid ethyl esters and phosphatidylethanols) in 50 μL of human whole blood. Pretreatment method, column and MS conditions were optimized. For the first time, the four types of ethanol non-oxidative metabolites with enormous discrepancies of property were simultaneously extracted and analyzed in one run within 40 min. The limits of detections (LODs) were among 0.1-10 ng/mL, and good linearity was obtained. Deviations in precision and accuracy were all lower than 15% at three QC levels. This method was then applied to two forensic samples, resulting in information on drinking habits and drinking time which were very useful for the interpretation of the blood alcohol results.
KW - Journal Article
U2 - 10.1016/j.aca.2017.01.040
DO - 10.1016/j.aca.2017.01.040
M3 - Journal article
C2 - 28335977
VL - 963
SP - 68
EP - 75
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -
ID: 174934818